Coding

Part:BBa_K2860000:Design

Designed by: Joy Barrett   Group: iGEM18_Bio_Without_Borders   (2018-08-07)


Limulus factor C


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2713
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 186
    Illegal BsaI.rc site found at 1631
    Illegal BsaI.rc site found at 1849


Design Notes

We removed all restriction enzyme sites and also codon optimized the protein sequence to go in a Pichia pastoris (yeast) vector.


Source

It comes from the genomic sequence of the Tachypleus tridentatus horseshoe crab species.

References